Kinetoplastids are a class of flagellated eukaryotic protists, including Trypanosoma and Leishmania, that threaten over 350 million people globally. Axonemal dynein is critical to the flagellar beat of T. brucei and has a central subunit, LC2, that we target. The role of LC2 in the function of dynein, and therefore in flagellar movement, has not been well characterized. Therefore, we designed guide RNAs (gRNAs) to program the CRISPR-Cas9 system to knock-out and tag LC2 homologs in T. brucei. gRNA leads the Cas9 endonuclease to different loci on the LC2 gene depending on the approach we wish to employ. To knock out the LC2 gene, we designed gRNA that targets and creates insertions and deletions in the middle of the gene. To tag LC2 with GFP, His6, and BCCP, we designed a gRNA that targets the end of the gene close to the 3’UTR using the Eukaryotic Pathogen CRISPR Guide RNA Design Tool, which reported no potential off target effects for the gRNA. Tagging will allow us to visualize LC2 in vitro and purify the axonemal dynein from trypanosome cells. The knock-out of the LC2 gene, with the efficiency of CRISPR-Cas9, will allow us to clearly quantify the role of LC2 in axonemal dynein and its impact on motility of T. brucei.The atypical flagellar beat observed in T. brucei can be better understood by applying CRISPR-Cas9 technology to the LC2 gene.